Jobs and Relevant Professional Positions

• Researcher in the area of animal reproduction of the National Institute of Livestock Research (INIP / INIFAP) in the Livestock Experimental Centers "La Posta" in Paso del Toro, See in dairy cattle and "Playa Vicente" in Lealtad de Muñoz, Playa Vicente , See. in zebu cattle.

• Regional Director of the Gulf of the Animal Reproduction Department of the National Institute of Livestock Research (INIP / INIFAP)

• National Director of the Animal Reproduction Department of the National Institute for Livestock Research (INIP / INIFAP).

• Founder and Director of various human fertility laboratories both in Mexico (Querétaro, Tijuana, Morelia) and in the United States (St. Louis, MO; Houston, TX).

• Advisor to private livestock companies involved in the transfer and production of in vitro embryos in the US, as well as in Mexico.

• Laboratory Inspector of the College of American Pathologists) in the USA

Professional Practical Experience

As a researcher and director in the Department of Animal Reproduction of INIP / INIFAP, I have accumulated vast experience in the management of dairy and beef cattle in the tropics using technological tools such as heat synchronization, artificial insemination, gamete and embryo management as well as in conducting and analyzing experiments to determine how the high humidity and hot environment of the tropics affected the reproductive performance of dairy cattle.

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In his doctoral studies, he carried out research on egg freezing for subsequent in vitro fertilization (IVF), being one of the first in the world to report the combination of two cryoprotectants used successfully to preserve frozen embryos.

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Later in his postdoc at the University of Missouri-Columbia he was in charge of mass producing thousands of in vitro bovine embryos to be used in other research studies in the laboratory of Dr. Michael R. Roberts. As a result of these activities, the following practical and scientific achievements were obtained:

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• To demonstrate for the first time using molecular techniques (RT-PCR) and bioassays the initiation of the synthesis of the bovine trophoblastic protein (b-TP1) now known as Interferon-Tau in embryos produced in-vitro. This protein's main function is to inhibit the production of uterine prostaglandins to prevent the destruction of the corpus luteum in the ovary and thus achieve the permanence and growth of the embryo.

• We were the first to successfully report the "sequential culture of embryos in-vitro" using the simple CZB medium in the first three days of embryo life followed by the TCM-199 complex medium. Subsequently, different commercial media arose in the market.

• Likewise, we were able to understand that the main objective of an in vitro embryo production system should focus mainly on obtaining a high percentage of embryos of 6 to 8 cells on day three to obtain a high proportion (>80%) of usable blastocysts.

• From the above, we created the following quality indicators or Key Performance Indicators (KPIs): >70% of embryos with 6 to 8 cells, <10% of 4 cells and <20% of embryos with 1 and 2 cells. Which are applied in a similar way both in humans and bovines.

• In a project for the production of transgenic embryos, we characterized the time of appearance of the male and female pronuclei (12-24 hours post insemination), with the highest incidence at 18 hours.

• The efficiency of different somatic cell lines (hepatic, connective, oviductal and renal) to be used in co-culture with embryos was determined.

• We demonstrate the inhibitory effect of complex media (TCM-199) on embryo development from day 1 to day 3 and the decrease in this effect as the proportion of a simple medium such as CZB increases.

• From all of the above, more than seven scientific publications and several book chapters arose.

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Professional Certifications

Certified by the American Board of Bioanalysis as Director of High Complexity Laboratories (HCLD), Clinical Consultant (CC).

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Scientific Publications

It has more than a hundred international scientific publications with an editorial committee, popular publications and producer magazines, as well as summaries in international meetings and book chapters.